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SIMULTANEOUS DIELECTROPHORETIC TRAPPING OF CELL ON OPPOSITE SIDES OF A PERMEABLE MEMBRANE

Published

Author(s)

Brian J. Nablo, Darwin R. Reyes-Hernandez

Abstract

Molecular transport through permeable membranes offers a unique opportunity to investigate cellular responses to nutrients, drugs, or toxins delivered to the culture’s apical and/or basal sides as well as cellular migration events. Herein, we present the means to rapidly localize and concentrate cells using dielectrophoresis (DEP) traps on opposing sides of a permeable membrane within a multilayer microfluidic device. This work establishes the possibility of generating on-chip cell co-cultures for high-throughput screening, similar to transwell chambers, but with the added benefits of the dynamic fluidic conditions from the microfluidic network and the rapid generation of cell cultures from DEP trapping.
Proceedings Title
Proceedings of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2015
Conference Dates
October 25-29, 2015
Conference Location
Gyeongyu, -1
Conference Title
The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2015

Keywords

Multilayer microfluidic system, co-culture, dielectrophoresis, permeable membrane
Created October 25, 2015, Updated February 19, 2017