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Search Publications by: Thomas Cleveland (Fed)

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Displaying 1 - 8 of 8

Characterization of AI Model Configurations For Model Reuse

October 24, 2022
Peter Bajcsy, Daniel Gao, Michael Paul Majurski, Thomas Cleveland, Manuel Carrasco, Michael Buschmann, Walid Keyrouz
With the widespread creation of artificial intelligence (AI) models in biosciences, bio-medical researchers are reusing trained AI models from other applications. This work is motivated by the need to characterize trained AI models for reuse based on

Ionization and Structural Properties of mRNA Lipid Nanoparticles that Influence Expression in Intramuscular and Intravascular Administration

August 11, 2021
Manuel Carrasco, Suman Alishetty, Mohamad-Gabriel Alameh, Hooda Said, Lacey Wright, Mikell Paige, Ousamah Soliman, Drew Weissman, Thomas Cleveland, Alexander Grishaev, Michael Buschmann
Lipid Nanoparticles (LNPs) are used to deliver siRNA and COVID-19 mRNA vaccines. The main factor known to determine their delivery efficiency is the pKa of the LNP containing an ionizable lipid. Herein, we report a method that can predict the LNP pKa from

Design of a novel, native-like secreted form of the hepatitis C virus E1E2 heterodimer

January 11, 2021
Johnathan D. Guest, Ruixue Wang, Khadija Elkholy, Andrezza Chagas, Kinlin L. Chao, Thomas Cleveland, Young C. Kim, Zhen-Yong Keck, Alexander Marin, Roy Mariuzza, Alexander K. Andrianov, Eric A. Toth, Steven Foung, Brian Pierce, Thomas R. Fuerst
Hepatitis C virus (HCV) is a major worldwide health burden, and a preventive vaccine is needed for global control or eradication of this virus. A substantial hurdle to an effective HCV vaccine is the high variability of the virus, leading to immune escape

Structure of the cell-binding component of the Clostridium difficile binary toxin reveals a di- heptamer macromolecular assembly

January 14, 2020
Xingjian Xu, Raquel Ruiz, Kaylin Adipietro, Christopher Peralta, Danya Ben-Hail, Kristen Varney, Mary Cook, Braden Roth, Paul Wilder, Thomas Cleveland, Alexander Grishaev, Heather Neu, Sarah Michel, Wenbo Yu, Dorothy Beckett, Richard Rustandi, Alex MacKerell, Amedee des Georges, Edwin Pozharski, David Weber
Targeting Clostridium difficile infection is challenging because treatment options are limited, and high recurrence rates are common. One reason for this is that hypervirulent C. difficile strains often have a binary toxin termed the C. difficile toxin, in

Small-Angle X-ray and Neutron Scattering Demonstrates that Cell-Free Expression Produces Properly Formed Disc-Shaped Nanolipoprotein Particles

December 20, 2017
Thomas E Cleveland, Wei He, Angela C. Evans, Nicholas O. Fischer, Matthew A. Coleman, Paul Butler
Nanolipoprotein particles (NLPs), composed of a membrane scaffold protein and lipids, have been used to support membrane proteins in a native-like bilayer environment for biochemical or structural studies. Traditionally, these NLPs have been prepared by

Isotopic Labeling of Proteins in Halobacterium salinarum

August 4, 2015
Thomas E. Cleveland, Zvi Kelman
It is often necessary to obtain isotopically labeled proteins containing 15N, 13C or 2H for NMR; and 2H for small-angle neutron scattering (SANS) or neutron diffraction studies. To achieve uniform isotopic labeling, protein expression is most commonly