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Displaying 26 - 38 of 38

Non-Uniform Sampling for All: More Spectral Quality, Less Measurement Time

June 30, 2017
Author(s)
Frank Delaglio, Gregory S. Walker, Kathleen A. Farley, Raman Sharma, Jeffrey C. Hoch, Luke Arbogast, Robert Brinson, John Marino
Nuclear Magnetic Resonance (NMR) spectroscopy is an indispensable tool in pharmaceutical science, with uses in drug discovery, development, and manufacturing. Non-Uniform Sampling (NUS) is an acquisition method for NMR experiments with two or more

Current and Emerging Technologies to Characterize the Higher-Order Structure of Monoclonal Antibodies

October 15, 2015
Author(s)
John Marino, Robert G. Brinson, Jane E. Ladner, David Travis Gallagher, Luke Arbogast, Richard Huang, Jeffrey W. Hudgens, Elyssia S. Gallagher
In contrast to small molecule therapeutics whose conformations can be absolutely defined by constitution and stereochemistry, biopharmaceuticals are distinguished by the requirement for folding into higher order structures (secondary, tertiary, and

2D 1HN, 15N Correlated NMR Methods at Natural Abundance for Obtaining Structural Maps and Statistical Comparability of Monoclonal Antibodies

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, Catherine A. Mouchahoir, James T. Hoopes, John Marino
Purpose High-resolution nuclear magnetic resonance spectroscopy (NMR) provides a robust approach for producing unique spectral signatures of protein higher order structure at atomic resolution. Such signatures can be used as a tool to establish consistency

Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, John Marino
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding can impact safety by eliciting unwanted immune or other off-target responses. Robust
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