Publications

Displaying 51 - 66 of 66

THE PROFICIENCY TEST (PILOT) REPORT OF THE GLOBAL MICROBIAL IDENTIFIER INITIATIVE YEAR 2014

March 1, 2016

Author(s)

Nathanael D. Olson, Rene S. Hendriksen, Susanne K. Pedersen, Mette V. Larsen, Jamie N. Pedersen, Oksana Lukjancenko, Rolf S. Kaas, Pimlapas Leekitcharoenphon, Lasse Bergmark, Inge M. Hansen, Vitali Sintchenko, William J. Wolfgang, Henrik T. Westh, Jacob Moran-Gilad, Isabel Cuesta, Angel Zaballos, Brian Beck, Anthony Underwood, Errol Strain, James Pettengill

Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

January 24, 2016

Author(s)

Sandra M. Da Silva, Lindsay Harris, Nathanael David Olson, Steven Lund, Autumn S. Downey, Zvi Kelman, Marc L. Salit, Jayne D. Morrow

Aims: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). Methods and results

Report from the Standards for Pathogen Identification via Next-Generation Sequencing (SPIN) Workshop

December 2, 2015

Author(s)

Nathanael D. Olson, Scott A. Jackson, Nancy J. Lin

Next-generation sequencing (NGS) is not routinely used in applied settings due to lack of confidence in results. This workshop convened experts to identify measurement challenges impeding NGS implementation and potential standards-based solutions to

Best Practices for Evaluating Single Nucleotide Variant Calling Methods for Microbial Genomics

July 7, 2015

Author(s)

Nathanael D. Olson, Steven P. Lund, Rebecca Colman, Jeffery T. Foster, Jason W. Sahl, James M. Schupp, Paul Keim, Jayne B. Morrow, Marc L. Salit, Justin M. Zook

Innovations in sequencing technologies have allowed biologists to make incredible advances in understanding biological systems. As experience grows, researchers increasingly recognize that analyzing the wealth of data provided by these new sequencing

Standards for Pathogen Identification via Next-Generation Sequencing (SPIN) Workshop Summary Report

June 1, 2015

Author(s)

Nathanael D. Olson, Nancy J. Lin, Scott A. Jackson

Advances in DNA sequencing over the last decade have lowered the barrier to whole genome sequencing. However, additional challenges must be overcome before widespread adoption of whole genome sequencing is realized for pathogen identification in applied

International Interlaboratory Study Comparing Single Organism 16S rRNA Sequencing Data: Going Beyond Consensus Sequence Comparisons

March 5, 2015

Author(s)

Nathanael D. Olson, Justin M. Zook, Steven P. Lund, Fabiola Rojas-Cornejo, Jim Huggett, Jayne B. Morrow

The Consultative Committee for Amount of Substance established a working group to advance the metrology of microbial identification and quantification. This study represents the initial step towards international comparability and measurement assurance for

Framework for the Evaluation of DNA Extract Suitability for Identification, Quantification and Sequencing of Unknown Microorganisms Microorganisms

December 3, 2012

Author(s)

Nathanael David Olson, Jayne D. Morrow

This study provides an evaluation of DNA extraction methods when tasked with identifying unknown microorganisms. Research, public health, and clinical laboratories frequently turn to DNA-based molecular methods to identify and quantify unknown

Optimization of processing method for recovery of bacteria from wipes used in biological surface sampling

August 3, 2012

Author(s)

Autumn S. Downey, Sandra M. Da Silva, Jayne B. Morrow, James J. Filliben, Nathanael D. Olson

Environmental sampling for microbiological contaminants is a key component of hygiene monitoring and risk characterization practices utilized across diverse fields of application. However, confidence in surface sampling results, both in the field and in

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