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Search Publications by: Robert Brinson (Fed)

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Displaying 26 - 37 of 37

Precision and Robustness of 2D-NMR for Structure and Assessment of Filgrastim Biosimilars

February 5, 2016
Author(s)
Houman Ghasriani, Derek Hodgson, Robert G. Brinson, Ian McEwen, Lucinda F. Buhse, Steven Kozlowski, John Marino, Yves Aubin, David Keire
The higher order structure of biotherapeutics is a critical quality attribute (CQA) that can be evaluated by nuclear magnetic resonance (NMR) spectroscopy at atomic resolution. NMR spectral "fingerprinting" can directly establish drug substance structural

Current and Emerging Technologies to Characterize the Higher-Order Structure of Monoclonal Antibodies

October 15, 2015
Author(s)
John Marino, Robert G. Brinson, Jane E. Ladner, David Travis Gallagher, Luke Arbogast, Richard Huang, Jeffrey W. Hudgens, Elyssia S. Gallagher
In contrast to small molecule therapeutics whose conformations can be absolutely defined by constitution and stereochemistry, biopharmaceuticals are distinguished by the requirement for folding into higher order structures (secondary, tertiary, and

Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as an Assignment Tool and Probe of Purine-rich DNA Dynamics Using Proton NMR Spectroscopy

June 30, 2015
Author(s)
Robert G. Brinson, Jennifer T. Miller, Kahn Jason, Stuart F. Le Grice, John P. Marino
Proton assignment of NMR spectra of oligonucleotide duplexes can become difficult with increasing helical length due to cross peak overlap. Here we describe an alternative to 15N and 13C labeling that facilitates assignment via single site substitution

2D 1HN, 15N Correlated NMR Methods at Natural Abundance for Obtaining Structural Maps and Statistical Comparability of Monoclonal Antibodies

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, Catherine A. Mouchahoir, James T. Hoopes, John Marino
Purpose High-resolution nuclear magnetic resonance spectroscopy (NMR) provides a robust approach for producing unique spectral signatures of protein higher order structure at atomic resolution. Such signatures can be used as a tool to establish consistency

Mapping Monoclonal Antibody Structure by 2D 13C NMR at Natural Abundance

March 1, 2015
Author(s)
Luke Arbogast, Robert G. Brinson, John Marino
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding can impact safety by eliciting unwanted immune or other off-target responses. Robust