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Search Publications by: David Travis Gallagher (Fed)

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Displaying 76 - 100 of 105

The Crystal Structure of Chorismate Lyase Shows a New Fold and a Tightly Retained Product

August 15, 2001
Author(s)
David T. Gallagher, M P. Mayhew, Marcia J. Holden, A J. Howard, K J. Kim, V L. Vilker
The enzyme chorismate lyase (CL) catalyzes the removal of pyruvate from chorismate to produce 4-hydroxy benzoate (4HB) for the ubiquinone pathway. In Escherichia coli, CL is monomeric with 164 residues; we have determined the structure of the CL product

Applying X-Ray Topography and Diffractometry to Improve Protein Crystal Growth

February 21, 2001
Author(s)
David R. Black, L Arnowitz, David T. Gallagher
In order to obtain adequate diffraction data to determine the structure of the protein of interest, crystal quality is important. Although in this context there may be no single concise definition of quality, it must involve adequate size, singleness

Role of Competitive Interactions in Growth Rate Trends of Subtilsin s88 Crystals

May 1, 2000
Author(s)
D Asthagirl, A Lenhoff, David Travis Gallagher
An orthorhombic crystal form of subtilisin BPN' variant s88 exhibits a systematic variation in growth rates of its three unique faces, resulting in pronounced variations in crystal morphology as a function of the ionic strength. We have sought to explain

Crystallization and 1.1 Angstrom Diffraction of Chorismate Lyase From Escherichia Coli

February 1, 2000
Author(s)
C Stover, M P. Mayhew, Marcia J. Holden, A Howard, David Travis Gallagher
Clorismate pathway enzymes are important as producers of nonnucleotide aromatic compounds. The enzyme chorismate layase from Escherichia coli has been crystallized in four distinct forms, three of which have been characterized by x-ray diffraction. Despite

Ultrasonic Measurement of Stress in Pin and Hanger Connections

January 1, 1999
Author(s)
A V. Clark, David T. Gallagher, C S. Hehman, P A. Fuchs, M G. Lozev
Pin and hanger connections are used in bridges to suspend an interior span from the outer spans. The connections can sometimes lock up due to corrosion. If lockup ocurs the stresses in the connection are cycled due to thermal expanison and contraction of