Publications

Displaying 1 - 25 of 108

Effects of Glycans and Hinge on Dynamics in the IgG1 Fc

October 28, 2023

Author(s)

Christina Bergonzo, J. Todd Hoopes, Zvi Kelman, David Travis Gallagher

The crystallizable fragment (Fc) domain of immunoglobulin subclass IgG1 antibodies is engineered for a wide variety of pharmaceutical applications. Two important structural variables in Fc constructs are the hinge region connecting the Fc to the antigen

SARS-CoV-2 infection establishes an enhanced, stable, and age-independent CD8+ T cell response against a dominant nucleocapsid epitope using highly restricted TCRs

October 23, 2023

Author(s)

Cecily Choy, Joseph Chen, Jiangyuan Li, Jian Lu, Daichao Wu, Ainslee Zou, Humza Hemani, Beverly Baptiste, Emily Wichmann, Qian Yang, Jeffrey Ciffelo, Rui Yin, Julia McKelvy, Denise Melvin, Tonya Wallace, Christopher Dunn, Cuong Nguyen, Chee Chia, Jingshui Fan, Jeannie Ruffolo, Linda Zukley, Guixin Shi, Tomokazu Amano, An Yang, Osorio Meirelles, Wells Wu, Rong-Fong Chen, RICHARD WILLIS, Minoru Ko, Y-T Liu, Supriyo De, Brian Pierce, Luigi Ferrucci, josephine egan, Roy Mariuzza, Nan-ping Weng, David Travis Gallagher

We analyzed circulating CD8+ T cells recognizing six epitopes from the SARS-CoV-2 nucleocapsid (N) protein in HLA-A2+ unexposed and recovered COVID-19 patients using multi-color flow cytometry and scRNAseq. We found that SARS-CoV-2 infection slightly

Monomeric Crystal Structure of the Vaccine Carrier Protein CRM197 and Implications for Vaccine Development

March 30, 2023

Author(s)

Andrew Lees, Natalia Oganesyan, David Travis Gallagher

CRM197 is a genetically detoxified mutant of diphtheria toxin (DT) that is widely used as a carrier protein for conjugate vaccines. Protective immune responses to H. influenzae b, S. pneumoniae, S. Typhi and meningococcal diseases are obtained by coupling

Design and characterization of a protein fold switching network

January 26, 2023

Author(s)

David Travis Gallagher, Biao Ruan, Yanan He, Yingwei Chen, Eun Jung Choi, Yihong Chen, Dana Motabar, Tsega Solomon, Richard Simmerman, Thomas Kauffman, John Orban, Philip Bryan

Protein sequences encoding three common small folds (3-alpha, beta-grasp, and alpha/beta plait) were connected in a network of mutational pathways that intersect at high-identity sequences, termed nodes. The structures of proteins around nodes were

Reversible switching between two common protein folds in a designed system using only temperature

January 19, 2023

Author(s)

Tsega Solomon, Yanan He, Fahriye Nese Sari, Yihong Chen, David Travis Gallagher, Philip Bryan, John Orban

Naturally occurring metamorphic proteins have the ability to interconvert from one folded state to another through either a limited set of mutations or by way of a change in the local environment. Here, we show that it is possible to switch reversibly

Atomic Model Structure of the NIST Monoclonal Antibody (NISTmAb) Reference Material

July 15, 2021

Author(s)

Christina Bergonzo, David Travis Gallagher

As monoclonal antibodies have become a vital resource in medicine, knowledge of their complex molecular structures has increased in importance. Thousands of antibody components (Fab and Fc fragments) are described in the Protein Data Bank. Whole antibodies

Crystal Structure of a Bivalent Antibody Fab Fragment

November 11, 2020

Author(s)

Salman Shahid, Mingming Gao, David Travis Gallagher, Steven Foung, Zhen-Yong Keck, Thomas Fuerst, Roy Mariuzza

We determined the crystal structure to 1.8 Å resolution of the Fab fragment of an affinity- matured human monoclonal antibody (HC84.26.5D) that recognizes the E2 envelope glycoprotein of hepatitis C virus (HCV). Unlike conventional Fabs, which are

Structural basis for oligoclonal T cell recognition of a shared p53 cancer neoantigen

June 9, 2020

Author(s)

David Travis Gallagher, Roy A. Mariuzza, Brian G. Pierce, Daichao Wu, Ragul Gowthaman

Abstract Adoptive cell therapy (ACT) with tumor-specific T cells can mediate cancer regression. The main target of tumor-specific T cells are neoantigens arising from mutations in self-proteins. Although the majority of cancer neoantigens are unique to

Structure and Dynamics of a Site-Specific Labeled Fc Fragment with Altered Effector Functions

October 21, 2019

Author(s)

David T. Gallagher, Robert G. Brinson, John P. Marino, nazzareno dimasi

Antibody-drug conjugates (ADCs) are a class of biotherapeutic drugs designed as targeted therapies for the treatment of cancer. Among the challenges in generating an effective ADC is the choice of an effective conjugation site on the IgG. One common method

Structure of the Fc Fragment from the NIST Reference Antibody RM8671

September 1, 2018

Author(s)

David Travis Gallagher, Connor V. Galvin, Ioannis Karageorgos

As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in

Data on crystal organization in the structure of the Fab fragment from the NIST reference antibody, RM 8671

December 1, 2017

Author(s)

David T. Gallagher, Ioannis L. Karageorgos, Jeffrey W. Hudgens, Connor V. Galvin

This article describes the crystal packing of the unliganded antibody fragment from the NIST reference antibody 8671, whose atomic coordinates are available as Protein Data Bank structure 5K8A [1]. Here we give information on the crystallization and

Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

December 1, 2017

Author(s)

Darion Spigolon, David Travis Gallagher, Adrian Velazquez-Campoy, Donatella Bulone, Jatin Narang, Pier San Biagio, Francesco Cappello, Alberto J. Macario, Everly Conway de Macario, Frank Robb

The human chaperonin complex is a 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy

Biophysical Characterization and Structure of the Fab Fragment from the NIST Reference Antibody RM 8671

September 29, 2017

Author(s)

Ioannis Karageorgos, Elyssia S. Gallagher, Connor Galvin, David Travis Gallagher, Jeffrey W. Hudgens

Monoclonal antibody (mAb) pharmaceuticals are the fastest-growing class of therapeutics with a wide range of clinical applications. To assure their safety, these protein drugs must demonstrate highly consistent purity and stability. Key to these objectives

Chorismate Lyase at 0.9 Resolution

February 19, 2017

Author(s)

David Travis Gallagher, A Howard

Chorismate

A Bacteriophage Endolysin that Eliminates Intracellular Streptococci

March 15, 2016

Author(s)

Yang Shen, Marilia Barros, Tarek Vennemann, David Travis Gallagher, Yizhou Yin, Sara B. Linden, Ryan D. Heselpoth, Dennis J. Spencer, David M. Donovan, John Moult, Vincent A. Fischetti, Frank Heinrich, Mathias Loesche, Daniel C. Nelson

PlyC, a bacteriophage-encoded A-B endolysin, lyses Streptococcus pyogenes (Spy) on contact and protects mice from upper respiratory Spy colonization. Here, we demonstrate that PlyC is a novel, potent agent for targeting and controlling intracellular Spy

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