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Search Publications by: David Travis Gallagher (Fed)

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Displaying 1 - 25 of 53

Effects of Glycans and Hinge on Dynamics in the IgG1 Fc

October 28, 2023
Christina Bergonzo, J. Todd Hoopes, Zvi Kelman, David Travis Gallagher
The crystallizable fragment (Fc) domain of immunoglobulin subclass IgG1 antibodies is engineered for a wide variety of pharmaceutical applications. Two important structural variables in Fc constructs are the hinge region connecting the Fc to the antigen

Design and characterization of a protein fold switching network

January 26, 2023
David Travis Gallagher, Biao Ruan, Yanan He, Yingwei Chen, Eun Jung Choi, Yihong Chen, Dana Motabar, Tsega Solomon, Richard Simmerman, Thomas Kauffman, John Orban, Philip Bryan
Protein sequences encoding three common small folds (3-alpha, beta-grasp, and alpha/beta plait) were connected in a network of mutational pathways that intersect at high-identity sequences, termed nodes. The structures of proteins around nodes were

Crystal Structure of a Bivalent Antibody Fab Fragment

November 11, 2020
Salman Shahid, Mingming Gao, David Travis Gallagher, Steven Foung, Zhen-Yong Keck, Thomas Fuerst, Roy Mariuzza
We determined the crystal structure to 1.8 Å resolution of the Fab fragment of an affinity- matured human monoclonal antibody (HC84.26.5D) that recognizes the E2 envelope glycoprotein of hepatitis C virus (HCV). Unlike conventional Fabs, which are

Structure of the Fc Fragment from the NIST Reference Antibody RM8671

September 1, 2018
David Travis Gallagher, Connor V. Galvin, Ioannis Karageorgos
As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in

Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

December 1, 2017
Darion Spigolon, David Travis Gallagher, Adrian Velazquez-Campoy, Donatella Bulone, Jatin Narang, Pier San Biagio, Francesco Cappello, Alberto J. Macario, Everly Conway de Macario, Frank Robb
The human chaperonin complex is a 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy

A Bacteriophage Endolysin that Eliminates Intracellular Streptococci

March 15, 2016
Yang Shen, Marilia Barros, Tarek Vennemann, David Travis Gallagher, Yizhou Yin, Sara B. Linden, Ryan D. Heselpoth, Dennis J. Spencer, David M. Donovan, John Moult, Vincent A. Fischetti, Frank Heinrich, Mathias Loesche, Daniel C. Nelson
PlyC, a bacteriophage-encoded A-B endolysin, lyses Streptococcus pyogenes (Spy) on contact and protects mice from upper respiratory Spy colonization. Here, we demonstrate that PlyC is a novel, potent agent for targeting and controlling intracellular Spy

Current and Emerging Technologies to Characterize the Higher-Order Structure of Monoclonal Antibodies

October 15, 2015
John Marino, Robert G. Brinson, Jane E. Ladner, David Travis Gallagher, Luke Arbogast, Richard Huang, Jeffrey W. Hudgens, Elyssia S. Gallagher
In contrast to small molecule therapeutics whose conformations can be absolutely defined by constitution and stereochemistry, biopharmaceuticals are distinguished by the requirement for folding into higher order structures (secondary, tertiary, and

Correction for stray light in optical spectroscopy of crystals

September 1, 2015
Richard W. Hendler, Curtis Meuse, David Travis Gallagher, Joerg Labahn, Jan Kubicek, Paul D. Smith, John W. Kakareka
It has long been known in spectroscopy that light not passing through a sample, but reaching the detector (i.e. stray light) results in a distortion of the spectrum known as absorption- flattening. In spectroscopy with crystals, one must either include

Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers

July 31, 2014
David J. Vanderah, Marlon L. Walker, David T. Gallagher, Ryan Vierling, Fay Crawshaw
Spectroscopic ellipsometry was used to evaluate the resistance to protein adsorption (RPA) of self- assembled monolayers (SAMs) of HS(CH2)3O(CH2CH2O)6M and [HS(CH2)3CH]2O-(CH2CH2O)6M, where M = CH3 or H, on Au. The SAMs were exposed to fibrinogen, a

On the need for an international effort to capture, share and use crystallisation screening data (failure and success)

June 1, 2012
David Travis Gallagher, Janet Newman, Evan E. Bolton, Jochen M?Dieckmann, Vincent J. Fazio, David Lovell, Joseph R. Luft, Thomas S. Peat, David Ratcliffe, Roger A. Sayle, Edward H. Snell, Kerry Taylor, Pascal Vallotton, Sameer Velankar, Frank V. Delft
Crystallisation of biological macromolecules is seen by most structural biologists as a necessary evil, a means to the end, which is knowledge about a macromolecular structure. Crystallization remains largely a trial-and-error process, with extensive

The Biological Macromolecule Crystallization Database

March 6, 2012
David Travis Gallagher, Michael Tung
The Biological Macromolecule Crystallization Database (BMCD) is described. The database currently contains 14372 entries with crystallization information for proteins, protein–protein complexes, nucleic acids, nucleic acid–nucleic acid complexes, protein

Protein crystal engineering of YpAC-IV using a strategy of excess charge reduction

August 5, 2009
David T. Gallagher, N N. Smith, Sung Kim, Howard Robinson, Prasad T. Reddy
The class IV adenylyl cyclase from Yersinia pestis has been engineered to enable crystallization at neutral pH for mechanism studies. The wild-type enzyme crystallized only at pH below 5. Based on the unliganded wild-type structure 2FJT at 1.9 Angstrom

A New Chemical Reactor for Protein Crystal Growth

October 16, 2008
David Travis Gallagher, C Stover, J Moses, D Charlton, E Steinberg, L Arnowitz
Current rapid advances in the genomic and biochemical sciences provide impetus for finding improved ways of crystallizing proteins for structure determination. A new dialysis-based reactor for protein crystal growth is described. The device utilizes motor

Structure of Archaeal Alanine Dehydrogenase and Relation to Bacterial and Human Proteins

October 16, 2008
David Travis Gallagher, H G. Monbouquette, I Schroeder, Hugh Robinson, Marcia J. Holden, N Smith
In the hyperthermophilic archaeon Archaeoglobus fulgidus, gene AF1665 encodes an L-ala dehydrogenase (AlaDH, EC but has been annotated as an ornithine cyclodeaminase (OCD) based on homology with the mu crystalline/ornithine cyclodeaminase protein