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Biotechnology

Biometrology

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Displaying 26 - 28 of 28

Regulation of Oxygen Level and Pervaporation in Microfluidic Devices

October 13, 2011
Author(s)
Peter C. Thomas, Srinivasa R. Raghavan, Samuel Forry
Oxygen level plays a critical role in maintaining cell homeostasis. Misregulation of oxygen levels in cell culture have been shown to impact cell growth, death and differentiation. As such, proper management of oxygen level is required in cell based assays

ON-CHIP PERVAPORATION-FREE CONTROL OF GAS PARTIAL PRESSURES

October 2, 2011
Author(s)
Peter C. Thomas, Laurie E. Locascio, Srinivasa R. Raghavan, Samuel Forry
Pre-equilibrated aqueous solutions were used on-chip to simultaneously mitigate pervaporation and modulate the partial pressures of oxygen (PO2) and carbon dioxide (PCO2) in stagnant culture chambers. This approach enabled long-term (>1 week), stopped-flow

Monitoring and Controlling Oxygen Levels in Microfluidic Devices

April 30, 2010
Author(s)
Samuel Forry, Peter C. Thomas, Srinivasa R. Raghavan
Mammalian cell culture has been traditionally performed in a static oxygen concentration of 20 mol %. However, oxygen level in vivo is significantly more hypoxic with an average oxygen concentration of 3 mol % to 5 mol %. In addition, many cells within the
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