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Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and
Molecular biosensors that accurately measure protein concentrations without external equipment are critical for solving numerous problems in diagnostics and therapeutics. Modularly transducing the binding of protein antibodies, protein switches or aptamers
Samuel Schaffter, Molly Wintenberg, Terence Murphy, Elizabeth Strychalski
Cotranscriptionally encoded RNA strand displacement (ctRSD) circuits are an emerging tool for programmable molecular computation, with potential applications spanning in vitro diagnostics to continuous computation inside living cells. In ctRSD circuits
Eugenia Romantseva, Nina Alperovich, David Ross, Steven Lund, Elizabeth Strychalski
DNA templates for protein expression remain an unexplored source of variability in the performance of cell-free expression (CFE) systems for protein production. To characterize this variability, we investigated the effects of two common DNA extraction
Engineered molecular circuits that process information in biological systems could address emerging human health and biomanufacturing needs. However, such circuits can be difficult to rationally design and scale. DNA-based strand displacement reactions
Eugenia Romantseva, Drew S. Tack, Nina Alperovich, David Ross, Elizabeth Strychalski
Cell-free systems are increasingly used for in vitro protein production and offer a promising alternative to traditional, cell-based biomanufacturing[1-6]. At the state-of-the-art, cell-free systems can provide a high-throughput platform for on-demand
The CELL-FREE Workshop sought to identify and prioritize actionable steps towards more reproducible and comparable cell-free systems for practical applications in bioengineering and biomanufacturing.
Thomas Cleveland, Wei He, Angela C. Evans, Nicholas O. Fischer, Matthew A. Coleman, Paul Butler
Nanolipoprotein particles (NLPs), composed of a membrane scaffold protein and lipids, have been used to support membrane proteins in a native-like bilayer environment for biochemical or structural studies. Traditionally, these NLPs have been prepared by