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Bioscience / Engineering / synthetic biology

Cell-free systems

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Displaying 1 - 8 of 8

Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report

November 20, 2024
Author(s)
Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and

Plug-and-play protein biosensors using aptamer-regulated in vitro transcription

September 12, 2024
Author(s)
Heonjoon Lee, Tian Xie, Byunghwa Kang, Xinjie Yu, Samuel Schaffter, Rebecca Schulman
Molecular biosensors that accurately measure protein concentrations without external equipment are critical for solving numerous problems in diagnostics and therapeutics. Modularly transducing the binding of protein antibodies, protein switches or aptamers

Effects of DNA Template Preparation on Variability in Cell-Free Protein Production

August 13, 2022
Author(s)
Eugenia Romantseva, Nina Alperovich, David Ross, Steven Lund, Elizabeth Strychalski
DNA templates for protein expression remain an unexplored source of variability in the performance of cell-free expression (CFE) systems for protein production. To characterize this variability, we investigated the effects of two common DNA extraction

Cotranscriptionally encoded RNA strand displacement circuits

March 23, 2022
Author(s)
Samuel Schaffter, Elizabeth Strychalski
Engineered molecular circuits that process information in biological systems could address emerging human health and biomanufacturing needs. However, such circuits can be difficult to rationally design and scale. DNA-based strand displacement reactions

Small-Angle X-ray and Neutron Scattering Demonstrates that Cell-Free Expression Produces Properly Formed Disc-Shaped Nanolipoprotein Particles

December 19, 2017
Author(s)
Thomas Cleveland, Wei He, Angela C. Evans, Nicholas O. Fischer, Matthew A. Coleman, Paul Butler
Nanolipoprotein particles (NLPs), composed of a membrane scaffold protein and lipids, have been used to support membrane proteins in a native-like bilayer environment for biochemical or structural studies. Traditionally, these NLPs have been prepared by
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