Edwards, D.
and Evans, R.
(2017),
Receptor heterogeneity in optical biosensors, Journal of Mathematical Biology, [online], https://doi.org/10.1007/s00285-017-1158-x, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=921791
(Accessed April 26, 2024)
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Receptor heterogeneity in optical biosensors
Published
Author(s)
David A. Edwards,
Abstract
Many biochemical reactions in nature involve a stream of chemical reactants (ligand molecules) flowing over a surface to which other reactants (receptors) are confined. Scientists measure rate constants associated with these reactions in an optical biosensor: an instrument in which ligand molecules are convected through a flow cell, over a surface to which other receptors are immobilized. Certain surface-receptor couplings have been shown to lead to a heterogenous receptor population on the sensor surface; this can obscure measurements of kinetic rate constants. We quantify the effects of a heterogenous receptor population on ligand binding, and derive a set of nonlinear differential equations which can be used to estimate rate constants from biosensor data.Citation
Journal of Mathematical Biology
Volume
76
Pub Type
Journals
Download Paper
Keywords
Biochemistry, Optical biosensors, Kinetic rate constants, Integrodifferential equations, Perturbation methods, Numerical methods
Citation
Created July 12, 2017, Updated October 12, 2021