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REALTIME UNCERTAINTY QUANTIFICATION VIA ULTRA-PRECISE PARTICLE MATCHING FOR HIGH-THROUGHPUT SERIAL CYTOMETRY

Published

Author(s)

Matthew DiSalvo, Paul Patrone, Gregory A. Cooksey

Abstract

A central paradigm in flow cytometry is the one-time optical interrogation of cells, a practice that has limited the ability to address important questions associated with reproducibility and repeatability of measurements. Serial flow cytometry has pioneered the quantification of measurement uncertainties by optically interrogating each object more than once along a flow path. Here, we address the throughput limitations of serial cytometry with a novel algorithm to match signals between different interrogation regions. The algorithm operated real-time in an automated microfluidic serial cytometer to achieve a tracking rate better than 99.95 % for event rates below 94 Hz.
Proceedings Title
The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Conference Dates
October 10-14, 2021
Conference Location
Palm Springs, CA, US

Keywords

Flow Cytometry, Microfluidics, Optofluidics, Reproducibility

Citation

DiSalvo, M. , Patrone, P. and Cooksey, G. (2021), REALTIME UNCERTAINTY QUANTIFICATION VIA ULTRA-PRECISE PARTICLE MATCHING FOR HIGH-THROUGHPUT SERIAL CYTOMETRY, The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, CA, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932659 (Accessed May 19, 2022)
Created September 27, 2021, Updated January 4, 2022