Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation
S Kulin, Rani B. Kishore, Joseph B. Hubbard, Kristian Helmerson
We report observation of real-time adhesion between a monoclonal IgE antibody and its specific DNP antigen. Both molecules are immobilized on the surfaces of different polystyrene microspheres trapped by optical tweezers. Monitoring spontaneous, thermally driven, successive attachment and detachment events allows a direct determination of the reaction limited detachment rate for a single bond and also for multiple bonds.