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Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation

Published

Author(s)

S Kulin, Rani B. Kishore, Joseph B. Hubbard, Kristian Helmerson

Abstract

We report observation of real-time adhesion between a monoclonal IgE antibody and its specific DNP antigen. Both molecules are immobilized on the surfaces of different polystyrene microspheres trapped by optical tweezers. Monitoring spontaneous, thermally driven, successive attachment and detachment events allows a direct determination of the reaction limited detachment rate for a single bond and also for multiple bonds.
Citation
Biophysical Journal
Volume
83
Issue
No. 4

Keywords

antigen, biological adhesion, IgE, optical tweezers, spontaneous dissociation

Citation

Kulin, S. , Kishore, R. , Hubbard, J. and Helmerson, K. (2002), Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation, Biophysical Journal (Accessed June 17, 2024)

Issues

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Created October 1, 2002, Updated February 17, 2017