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Quantitation of Laminin Adsorbed onto Flexible Substrates Using Various Treatment Protocols

Published

Author(s)

Hae-Jeong Lee, Marvi Matos, Lisa Pakstis, Marcus T. Cicerone, Joy Dunkers

Abstract

There is considerable interest in how cells respond to mechanical stimuli, from the ligands used to transmit the stimulus to the signaling pathways initiated to the proteins expressed upon phenotype change. Our work focuses on using a flexible substrate treated with extracellular matrix (ECM) to induce tensile strain on cells. It is the evaluation of the quality of the ECM coating and cell proliferation that was the focus of previous work. In short, we subjected PDMS substrates to oxidation via plasma and one of the following treatments using fibronectin or laminin: physical adsorption, adsorption via polar interactions (chemisorption), and covalent bonding. Physical characterization of relative amounts of protein coverage, uniformity, roughness and hydrophilicity was performed. Attachment and proliferation of rat aortic smooth muscle (A10) cells were also evaluated.
Proceedings Title
Proceedings of the ASME 2008 Summer Bioengineering Conference
Conference Dates
June 25-29, 2008
Conference Location
Marco Island, FL, US

Keywords

smooth muscle cells, biomechanics, polydimethylsiloxane, X-ray reflectivity

Citation

Lee, H. , Matos, M. , Pakstis, L. , Cicerone, M. and Dunkers, J. (2008), Quantitation of Laminin Adsorbed onto Flexible Substrates Using Various Treatment Protocols, Proceedings of the ASME 2008 Summer Bioengineering Conference, Marco Island, FL, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=854427 (Accessed June 5, 2023)
Created June 24, 2008, Updated October 12, 2021