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Quantification of Inflammatory Cellular Responses Using Real-Time Polymerase Chain Reaction

Published

Author(s)

L A. Bailey, N. R. Washburn, Carl G. Simon Jr., E S. Chan, Francis W. Wang

Abstract

: The introduction of tissue engineering approaches for the repair and replacement of human body components extends the application and importance of synthetic materials (biomaterials). Implanted biomaterials frequently evoke inflammatory responses, and are subsequently responsible for implant failure. We have established a system that allows for the analysis and quantitation of cellular inflammatory responses in vitro using Real- Time Polymerase Chain Reaction (RT-PCR). The inflammatory responses of non-transformed murine macrophages (Raw 264.7) cells were analyzed after incubation with polymethylmethacrylate (PMMA) microspheres in the presence and absence of lipopolysaccharide (LPS) at 8hrs. Elevated cytokine production serves as an indicator of inflammatory responses, thus we monitored the levels of interlukin-1b produced. Furthermore, we demonstrate that the presence of a bacterial component and debris particles can work in concert to elevate cytokine production leading to inflammation.
Citation
Journal of Biomedical Materials Research Part A
Volume
69A
Issue
No. 2

Keywords

biomaterials, inflammation

Citation

Bailey, L. , Washburn, N. , Simon, C. , Chan, E. and Wang, F. (2004), Quantification of Inflammatory Cellular Responses Using Real-Time Polymerase Chain Reaction, Journal of Biomedical Materials Research Part A, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852171 (Accessed April 19, 2024)
Created May 1, 2004, Updated February 19, 2017