A Proteomics Performance Standard to Support Measurement Quality in Proteomics

Published

April 21, 2012

Author(s)

Ashley B. Green, David M. Bunk, Paul A. Rudnick, Karen W. Phinney, Lisa E. Kilpatrick

Abstract

The emergence of mass spectrometry-based proteomic platforms as a prominent technology utilized in biochemical and biomedical research has increased the need for high-quality MS measurements. To address this need, NIST Reference Material (RM) 8323 Yeast Protein Extract is introduced as a proteomics quality control material for benchmarking the pre-analytical and analytical performance of proteomics-based experimental workflows. RM 8323 Yeast Protein Extract is based upon the well-characterized eukaryote Saccharomyces cerevisiae and can be utilized in the design and optimization of proteomics-based methodologies from sample preparation to data analysis. To demonstrate its utility as a proteomics quality control material, we coupled LC-MS/MS measurements of RM 8323 with the NIST MSQC performance metrics to quantitatively assess the LC-MS/MS instrumentation parameters that influence measurement accuracy, repeatability and reproducibility. Due to the complexity of the yeast proteome, we also demonstrate how NIST RM 8323, along with the NIST MSQC performance metrics, can be used in the evaluation and optimization of proteomics-based sample preparation methods.

Citation

Proteomics

Pub Type

Journals

Keywords

Liquid chromatography-Tandem MS, Proteomics, Quality Control Material and Reference Material

Citation

Green, A. , Bunk, D. , Rudnick, P. , Phinney, K. and Kilpatrick, L. (2012), A Proteomics Performance Standard to Support Measurement Quality in Proteomics, Proteomics (Accessed December 11, 2024)