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Preparation and Comprehensive Characterization of a Calicum Hydroxyapatite Reference Material



D W. Fowler, Milenko Markovic, Michael Tung


Numerous biological and chemical studies involve the use of calcium hydroxyapatite Ca10(PO4)6(OH)2, HA. In this study detailed physicochemical characterization of HA prepared from an aqueous solution was carried out employing different methods and techniques: chemical and thermal analyses, X-ray diffraction, infrared and Raman spectroscopies, scanning and transmission microscopies and Brunauer, Emmett, and Teller (BET) surface-area method. The Ca, PO4, OH, HPO4, H2O, CO3, and trace constituent contents, the Ca/P molar ratio, crystal size and morphology, surface area, unit-cell parameters, crystallinity, and solubility of this HA were determined. This homogeneous, essentially pure and crystalline HA is certified as a National Institute of Standards and Technology (NIST) standard reference material (SRM 2910).
Journal of Research (NIST JRES) -
109 No. 6


chemical analysis, crystal size, crystallinity, hydroxyapatite, infrared, morphology, preparation, Raman, surface area


Fowler, D. , Markovic, M. and Tung, M. (2004), Preparation and Comprehensive Characterization of a Calicum Hydroxyapatite Reference Material, Journal of Research (NIST JRES), National Institute of Standards and Technology, Gaithersburg, MD (Accessed April 17, 2024)
Created October 31, 2004, Updated October 12, 2021