Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Practical Considerations for Preparing Polymerized Phospholipid Bilayer Capillary Coatings for Protein Separations

Published

Author(s)

Elisabeth Mansfield, Seid M. Adem, John P. Keogh, Craig A. Aspinwall, Henry K. Hall

Abstract

Phosphorylcholine (PC) based phospholipid bilayers have proven useful as capillary coating materials due to their inherent resistance to non-specific protein adsorption. The primary limitation of this important class of capillary coatings remains the limited long-term chemical and physical stability of the coatings. Recently, a method for increasing phospholipid coating stability in fused silica capillaries via utilization of polymerized, synthetic phospholipids was reported. Here, we expand upon these studies by investigating polymerized lipid bilayer capillary coatings with respect to separation performance including run-to-run, day-to-day and column-to-column reproducibility and long term stability. In addition, the effects of pH and capillary inner diameter on polymerized phospholipid coated capillaries were investigated to identify optimized coating conditions. The coatings are stabilized for protein separations across a wide range of pH values (4.0-9.3), a unique property for capillary coating materials. Additionally, smaller inner diameter capillaries (≤ 50 m) were found to yield marked enhancements in coating stability and reproducibility compared to wider bore capillaries, demonstrating the importance of capillary size for separations employing polymerized phospholipid coatings.
Citation
Analytica Chimica ACTA

Keywords

capillary electrophoresis, phospholipids, separations

Citation

Mansfield, E. , Adem, S. , Keogh, J. , Aspinwall, C. and Hall, H. (2013), Practical Considerations for Preparing Polymerized Phospholipid Bilayer Capillary Coatings for Protein Separations, Analytica Chimica ACTA (Accessed April 19, 2024)
Created April 13, 2013, Updated February 19, 2017