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Poly(ethylene glycol)s 2000-8000 in Water May be Planar: A Small-Angle Neutron Scattering (SANS) Structure Study
Published
Author(s)
Kenneth A. Rubinson, Susan Krueger
Abstract
The low-resolution three-dimensional structure of purified beef heart mitochondrial cytochrome c oxidase in asolectin unilameler liposomes has been measured by small-angle neutron scattering under conditions where the liposome scattering is minimized (contrast-match conditions). Modeled as a simple parallelpiped, the dimer has dimensions of 5.6 × 6.1 × 11.3 nm. (uncertainties, respectively, 1.1, 1.1, 1.8 nm). The molecular mass calculated for a typical protein with this volume will be (320 ± 95) kDa; the mass indicates a dimer of the protein. Since the long dimension has some interference from intermolecular scattering, it may be estimated using an average protein density and the known dimer molecular mass. This estimated length is 14.5 nm with the same cross section. The measured scattering curve of the dimer differs significantly from that calculated from the x-ray structure of the dimer in a crystal of mixed micelles (PDB 3AG1)
Membrane protein structure, SANS, cytochrome c oxidase, liposome
Citation
Rubinson, K.
and Krueger, S.
(2009),
Poly(ethylene glycol)s 2000-8000 in Water May be Planar: A Small-Angle Neutron Scattering (SANS) Structure Study, Polymer, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=908655
(Accessed October 10, 2024)