Phase mask-based multimodal superresolution microscopy

Published

July 6, 2017

Author(s)

Ryan Beams, Jeremiah W. Woodcock, Jeffrey W. Gilman, Stephan J. Stranick

Abstract

We demonstrate a multimodal superresolution microscopy technique based on a phase masked excitation beam in combination with spatially filtered detection. The theoretical foundation for calculating the focus from a non-paraxial beam with an arbitrary azimuthally symmetric phase mask is presented for linear and two-photon excitation processes as well as the theoretical resolution limitations. Experimentally this technique is demonstrated using two- photon luminescence from 80 nm gold particle as well as two-photon fluorescence lifetime imaging of fluorescent polystyrene beads. Finally to illustrate the versatility of this technique we acquire two-photon fluorescence lifetime, two-photon luminescence, and second harmonic images of a mixture of fluorescent molecules and 80 nm gold particles with

Citation

Optica

Volume

4

Issue

3

Pub Type

Journals

Download Paper

https://doi.org/10.3390/photonics4030039

Keywords

microscopy, superresolution, nonlinear microscopy

Citation

Beams, R. , Woodcock, J. , Gilman, J. and Stranick, S. (2017), Phase mask-based multimodal superresolution microscopy, Optica, [online], https://doi.org/10.3390/photonics4030039 (Accessed October 17, 2025)