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Parametric optimization of an air-liquid interface system for flow through inhalation exposure to nanoparticles: assessing dosimetry and intracellular uptake of CeO2 nanoparticles

Published

Author(s)

Lars Leibrock, Harald Jungnickel, Jutta Tentschert, Aaron Katz, Blaza Toman, Elijah Petersen, Frank Bierkandt, Ajay V. Singh, Peter Laux, Andreas Luch

Abstract

Air-liquid interface (ALI) systems have been widely used in recent years to investigate the inhalation toxicity of many gaseous compounds, chemicals, and nanomaterials and represent an emerging and promising in vitro method to supplement or ultimately replace in vivo studies. In general, the comparability of the toxicological results obtained from in vivo and in vitro inhalation experiments is still challenging. The ALI exposure setup, mirroring the physiological conditions of the deep lung (i.e., alveoli tissue), more closely resembles a subacute in vivo inhalation exposure scenario compared to ubmerged culture exposure. However, the robustness of the ALI exposure scenario is not yet well understood, but critical for the potential standardization of these methods. We investigated the influence of five different instrumental and physiological parameters affecting cell viability and exposure parameters of a human lung cell line: exposure duration, relative humidity, temperature, CO2 concentration, and flow rate. Exposing lung epithelia cells to a CeO2 nanoparticle-containing aerosol using this method, intracellular CeO2 concentrations were similar to those found in a recent subacute inhalation study in rats. To our knowledge, this is the first time showing that the nanoparticle (NP) concentration reached in vitro using a flow through ALI system were the same as those obtained in an in vivo study.
Citation
Nanomaterials

Citation

Leibrock, L. , Jungnickel, H. , Tentschert, J. , Katz, A. , Toman, B. , Petersen, E. , Bierkandt, F. , Singh, A. , Laux, P. and Luch, A. (2021), Parametric optimization of an air-liquid interface system for flow through inhalation exposure to nanoparticles: assessing dosimetry and intracellular uptake of CeO2 nanoparticles, Nanomaterials, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=930921 (Accessed December 8, 2022)
Created September 29, 2021, Updated October 14, 2021