Osteoblast response to polymeric composites varying in composition, conversion and roughness using a combinatorial approach

Published

June 10, 2009

Author(s)

Nancy J. Lin, Sheng Lin-Gibson

Abstract

Dimethacrylates typically used in dental composites are seeing increased applications in orthopedics, applications where cell-material contact is highly likely and often encouraged. Some formulations of dimethacrylate polymers and composites have been shown to be toxic, so direct contact cytotoxicity assays should be used to assess the toxicity of these materials for orthopedic applications. This study utilized our combinatorial testing platform to evaluate the cell response to dimethacrylate composites with a variety of properties on a single sample. MC3T3-E1 pre-osteoblasts were cultured directly on composites with varying filler content, filler type, degree of conversion (DC), and surface topography. Cell viability, density, and area depended on an interplay of the material properties, with low DC causing a reduction in cell area but having minimal effect on cell viability, high filler content causing an increase in cell density, and filler content/type altering the surface roughness as a function of DC. The combinatorial testing platform successfully quantified the effects of numerous material properties on several aspects of the osteoblast response.

Citation

Biomaterials

Volume

30

Issue

27

Pub Type

Journals

Download Paper

DOI Link

Keywords

Biomaterial, cell response, composite, contact angle, cytotoxicity, dimethacrylate, gradient, osteoblast, photopolymerization, surface topography

Citation

Lin, N. and Lin-Gibson, S. (2009), Osteoblast response to polymeric composites varying in composition, conversion and roughness using a combinatorial approach, Biomaterials, [online], https://doi.org/10.1016/j.biomaterials.2009.05.019 (Accessed April 25, 2024)