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Ongoing Development of Two-Dimensional Polyacrylamide Gel Electrophoresis Data Standards



Veerasamy Ravichandran, Joshua Lubell, G B. Vasquez, P Lemkin, Ram D. Sriram, G L. Gilliland


As the amount of proteomics data in the public databases grow, the volume of information available to the scientific community is constantly increasing and diversifying. Two-Dimensional Polyacrylamide Gel Electrophoresis (2-D PAGE) is a common method used to fractionate and identify proteins. Information related to 2-D PAGE are scattered in many different data sources. Most of 2-D PAGE experimental details are naturally addressed and are context dependent. One of the principal barriers for 2-D PAGE data interaction lies in the complex and varied vocabulary used by biologists to define the attribute of a molecule. Without a universally acceptable, common protocol, it is very difficult to understand data or representations from different sources. Defining data standards for each data item is thus the first step towards the uniformity of 2-D PAGE data and 2-D PAGE data interoperability. The current focus is on establishing standards for 2-D PAGE experimental data annotation and data exchange/interoperability, subsequently facilitating the creation of a 2-D PAGE experimental data repository. We propose here to have a common language for sharing electrophoresis experimental data, Two-dimensional Electrophoresis Markup Language (TWODML) that is based on the XML.


data repository, data standards, markup language, Proteomics, two-dimensional electrophoresis


Ravichandran, V. , Lubell, J. , Vasquez, G. , Lemkin, P. , Sriram, R. and Gilliland, G. (2004), Ongoing Development of Two-Dimensional Polyacrylamide Gel Electrophoresis Data Standards, Electrophoresis, [online], (Accessed May 25, 2024)


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Created January 1, 2004, Updated February 17, 2017