Published: August 31, 2018
Alexander V. Grishaev, Robert Best, Alessandro Borgia, Wenwei Zheng, Karin Buholzer, Madeleine Borgia, Hagen Hofmann, Daniel Nettels, Klaus Gast, Benjamin Schuler
The degree of compaction inferred from SAXS experiments by Riback et al. for unfolded proteins in water versus chemical denaturant is highly consistent with the results from FRET experiments. There is thus no contradiction between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction.
Pub Type: Journals
SAXS, FRET, IDP, intrinsically disordered proteins, denaturation
Created August 31, 2018, Updated May 01, 2019