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Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics

Published

Author(s)

Joseph E. Reiner, John J. Kasianowicz, Joseph W. Robertson

Abstract

Polyethylene glycol (PEG) is a ubiquitous charge-neutral polymer having several useful properties. It has been used to estimate the diameter of functioning nanopores. In this talk I will describe how the detailed analysis of PEG induced current blockades from a single alpha hemolysin channel in an unsupported lipid bilayer can be used to estimate step-wise changes in the structure of a functioning alpha hemolysin nanopore to < 1 Å. I will also describe a thermodynamic model that estimates the binding strength of cations to single PEG molecules from both the magnitude of PEG induced current blockades and the mean residence time of PEG within the nanopore.

Citation

Reiner, J. , Kasianowicz, J. and Robertson, J. (2010), Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics (Accessed June 23, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created August 23, 2010, Updated October 12, 2021