An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics
Published
Author(s)
Joseph E. Reiner, John J. Kasianowicz, Joseph W. Robertson
Abstract
Polyethylene glycol (PEG) is a ubiquitous charge-neutral polymer having several useful properties. It has been used to estimate the diameter of functioning nanopores. In this talk I will describe how the detailed analysis of PEG induced current blockades from a single alpha hemolysin channel in an unsupported lipid bilayer can be used to estimate step-wise changes in the structure of a functioning alpha hemolysin nanopore to < 1 Å. I will also describe a thermodynamic model that estimates the binding strength of cations to single PEG molecules from both the magnitude of PEG induced current blockades and the mean residence time of PEG within the nanopore.
Reiner, J.
, Kasianowicz, J.
and Robertson, J.
(2010),
Nanopore-based Single Molecule Determination of Polymer-cation Binding and Membrane Protein Dynamics
(Accessed November 29, 2023)