Multiplexed SERS Detection of Serum Cardiac Biomarkers Using Plasmonic Metasurfaces

Published

October 15, 2024

Author(s)

Peng Zheng, Lintong Wu, Piyush Raj, Jeong Hee Kim, Santosh Paidi, Stephen Semancik, Ishan Barman

Abstract

Surface-enhanced Raman spectroscopy (SERS) possesses exquisite molecular-specific properties with single-molecule sensitivity. Yet, translation of SERS into a quantitative analysis technique remains elusive owing to considerable fluctuation of the SERS intensity, which can be ascribed to the SERS uncertainty principle, a tradeoff between "reproducibility" and "enhancement". To provide a potential solution, herein, we propose an integrated multiplexed SERS biosensing strategy, which features two distinct advantages. First, a subwavelength-structured plasmonic metasurface consisting of alternately stacked metal-dielectric pyramidal meta-atoms is fabricated and can provide simultaneously enhanced electric and magnetic fields to enable spatially extended and weakly wavelength-dependent SERS. Second, nanomechanical perturbations are harnessed to transduce signals in the form of SERS frequency shifts, which are not directly affected by the SERS uncertainty principle. By also employing 3D printing methods, we provide a proof-of-concept study of multiplexed detection of a panel of serum cardiac biomarkers for acute myocardial infarction. We envision that success in the development of both the electric and magnetic fields-active plasmonic metasurfaces could transform future designs of SERS substrates with newly endowed functionalities, and that frequency shift-based SERS multiplexing could open new opportunities to develop innovative quantitative optical techniques for applications in chemistry, biology, and medicine.

Citation

Advanced Functional Materials

Pub Type

Journals

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https://doi.org/10.1002/advs.202405910

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Keywords

plasmonic metasurface, Raman spectroscopy, SERS, multiplexed detection, cardiac biomarkers

Citation

Zheng, P. , Wu, L. , Raj, P. , Kim, J. , Paidi, S. , Semancik, S. and Barman, I. (2024), Multiplexed SERS Detection of Serum Cardiac Biomarkers Using Plasmonic Metasurfaces, Advanced Functional Materials, [online], https://doi.org/10.1002/advs.202405910, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=957444 (Accessed September 13, 2025)