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Microfluidic Analysis of complex samples with minimal sample preparation using Gradient Elution Moving Boundary Electrophoresis

Published

Author(s)

Elizabeth A. Strychalski, Alyssa Henry, David J. Ross

Abstract

Sample-in answer-out analytical tools remain the goal of much lab on a chip research, but miniaturized methods capable of examining minimally prepared samples have proven elusive. Complex samples, including whole milk, various types of dirt and leaves, coal fly ash, and blood serum were analyzed quantitatively for dissolved potassium, calcium, sodium, magnesium, lithium, and melamine using gradient elution moving boundary electrophoresis (GEMBE) and contactless conductivity detection with the single preparatory step of dilution or suspension in sample buffer. GEMBE is a simple, robust analytical technique well-suited to microfluidic analysis of complex samples containing material, such as particulates or proteins, that would confound the majority of other microfluidic techniques. GEMBE utilizes electrophoretic flow to drive electrically charged analytes into a microfluidic channel or capillary for detection, while opposing electroosmotic and variable pressure-driven flows prevent the remainder of the sample from entering the capillary. Contactless conductivity detection further simplifies device construction and operation, positioning GEMBE for inexpensive and facile multiplexed implementation outside laboratory settings.
Citation
Analytical Chemistry
Volume
81
Issue
24

Citation

Strychalski, E. , Henry, A. and Ross, D. (2009), Microfluidic Analysis of complex samples with minimal sample preparation using Gradient Elution Moving Boundary Electrophoresis, Analytical Chemistry (Accessed April 18, 2024)
Created December 15, 2009, Updated February 19, 2017