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MICROFABRICATION OF A 3D, ELASTIC CELL MICROENVIRONMENT WITH NANOSCALE PRECISION USING LAYER-BY- LAYER DEPOSITION

Published

Author(s)

Markela Ibo, Kiran Bhadriraju, Darwin Reyes-Hernandez

Abstract

Here we present a unique method to generate a customized cell microenvironment that allows for the positioning of specific components of the extracellular matrix, and other polyelectrolytes, at a precise distance from the matrix surface; thus, providing a variable, well-coordinated material that could be tailored for essential cell-surface interactions. Weakly cross-linked melamine formaldehyde (MF) microparticles were packed in microfluidic channels and polyelectrolyte multilayers were formed, layer-by-layer, on top of them. The MF microparticles were then dissolved and matrices with an elastic modulus of 10 kPa, and capable of maintaining cells alive for at least 24 h, were obtained.
Proceedings Title
Proceedings of Micro Total Analysis Systems 2017
Conference Dates
October 22-26, 2017
Conference Location
Savannah, GA, US
Conference Title
The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2017
Conference

Keywords

Layer-by-layer deposition, 3D-like cell culture matrix, laminin, fibronectin, polyelectrolytes

Citation

Ibo, M. , Bhadriraju, K. and Reyes-Hernandez, D. (2017), MICROFABRICATION OF A 3D, ELASTIC CELL MICROENVIRONMENT WITH NANOSCALE PRECISION USING LAYER-BY- LAYER DEPOSITION, Proceedings of Micro Total Analysis Systems 2017, Savannah, GA, US (Accessed January 28, 2023)
Created October 21, 2017, Updated October 12, 2021