MICROFABRICATION OF A 3D, ELASTIC CELL MICROENVIRONMENT WITH NANOSCALE PRECISION USING LAYER-BY- LAYER DEPOSITION

Published: October 22, 2017

Author(s)

Markela Ibo, Kiran Bhadriraju, Darwin R. Reyes-Hernandez

Abstract

Here we present a unique method to generate a customized cell microenvironment that allows for the positioning of specific components of the extracellular matrix, and other polyelectrolytes, at a precise distance from the matrix surface; thus, providing a variable, well-coordinated material that could be tailored for essential cell-surface interactions. Weakly cross-linked melamine formaldehyde (MF) microparticles were packed in microfluidic channels and polyelectrolyte multilayers were formed, layer-by-layer, on top of them. The MF microparticles were then dissolved and matrices with an elastic modulus of ~10 kPa, and capable of maintaining cells alive for at least 24 h, were obtained.
Proceedings Title: Proceedings of Micro Total Analysis Systems 2017
Conference Dates: October 22-26, 2017
Conference Location: Savannah, GA
Conference Title: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, ┬ÁTAS 2017 Conference
Pub Type: Conferences

Keywords

Layer-by-layer deposition, 3D-like cell culture matrix, laminin, fibronectin, polyelectrolytes
Created October 22, 2017, Updated September 20, 2018