Gallagher, D.
, Pan, Q.
and Gilliland, G.
(1998),
Mechanism of Ionic Strength Dependence of Crystal Growth Rates in a Subtilsin Variant, Journal of Crystal Growth
(Accessed May 3, 2025)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Mechanism of Ionic Strength Dependence of Crystal Growth Rates in a Subtilsin Variant
Published
Author(s)
, Q Pan,
Abstract
An orthorhombic crystal form of subtilisin exhibits a systematic variation in growth rates of its three unique faces, resulting in pronounced morphology variations, depending on ionic strength. Several common salts cause a concentration-dependent change in the crystal habit from thin plates to isometric bars. The effect is analyzed in terms of the four crystal contacts in the 1.75 A resolution X-ray structure. Morphological growth fronts and the rate-limiting attachment step for growth of each face are considered, and it is concluded that ion screening of a carboxyl-carboxyl interaction in one crystal contact plays a key role in the salt-morphology effect.Citation
Journal of Crystal Growth
Volume
193
Issue
4
Pub Type
Journals
Keywords
crystal structure, ion screening, lattice packing, protein crystal growth, subtilisin
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.
Created October 1, 1998, Updated February 19, 2017