Evans, R.
, Edwards, D.
and Li, W.
(2017),
Measuring kinetic rate constants of multiple-component reactions with optical biosensors, Analytical Biochemistry, [online], https://doi.org/10.1016/j.ab.2017.06.009
(Accessed December 11, 2024)
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Measuring kinetic rate constants of multiple-component reactions with optical biosensors
Published
Author(s)
, David A. Edwards, Wenbin Li
Abstract
One may measure the kinetic rate constants associated with biochemical reactions using an optical biosensor: an instrument in which ligand molecules are convected through a flow cell over a surface to which receptors are immobilized. If there are multiple reactants, one is faced with the problem of fitting multiple kinetic rate constants to one signal, since data from all of the reacting species is lumped together. We show that even in the presence of ambiguous data, we may use a series of experiments to accurately determine the rate constants. Moreover, we show that we may also identify the true set of rate constants by either postprocessing the signals or adjusting the ligand inflow concentrations.Citation
Analytical Biochemistry
Volume
533
Pub Type
Journals
Download Paper
Keywords
Optical biosenors, Kinetic rate constants
Citation
Issues
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Created September 14, 2017, Updated September 27, 2020