Measuring kinetic rate constants of multiple-component reactions with optical biosensors

Published

September 15, 2017

Author(s)

Ryan M. Evans, David A. Edwards, Wenbin Li

Abstract

One may measure the kinetic rate constants associated with biochemical reactions using an optical biosensor: an instrument in which ligand molecules are convected through a flow cell over a surface to which receptors are immobilized. If there are multiple reactants, one is faced with the problem of fitting multiple kinetic rate constants to one signal, since data from all of the reacting species is lumped together. We show that even in the presence of ambiguous data, we may use a series of experiments to accurately determine the rate constants. Moreover, we show that we may also identify the true set of rate constants by either postprocessing the signals or adjusting the ligand inflow concentrations.

Citation

Analytical Biochemistry

Volume

533

Pub Type

Journals

Download Paper

https://doi.org/10.1016/j.ab.2017.06.009

Local Download

Keywords

Optical biosenors, Kinetic rate constants

Citation

Evans, R. , Edwards, D. and Li, W. (2017), Measuring kinetic rate constants of multiple-component reactions with optical biosensors, Analytical Biochemistry, [online], https://doi.org/10.1016/j.ab.2017.06.009 (Accessed April 26, 2024)