lSPR Study of DNA Wrapped Single Wall Carbon Nanotube (ssDNA-SWCNT) Adsorption on a Model Biological (Collagen) Substrate
Jung Jin Park, Jeffrey Fagan, JiYeon Huh, Kalman D. Migler, Alamgir Karim, Dharmaraj Raghavan
The kinetics of single stranded-DNA dispersed single wall carbon nanotubes (SWCNTs) adsorption onto an immobilized collagen layer in a microfluidic channel was probed using surface plasmon resonance (SPR) imaging. The adsorption was measured for a range of both nanoparticles and solution parameters including the nanotube concentration, nanotube length, solution pH and the type of medium. The kinetic adsorption data suggests that the adsorption of the nanotubes to the collagen layer is irreversible in HEPES buffer, pH ≈ 7.4, at room temperature, with the nanotube adsorption displaying two different adsorption kinetics regimes for different concentrations. Diffusion-limited processes were predominant at lower concentrations (< 40 g/mL) while the adsorption was governed by a reaction-limited process at higher concentration (> 40 g/mL). Adsorption measurements as a function of nanotube length also displayed differences in the apparent adsorption processes. Adsorption of shorter nanotubes ( 40 nm) was found to follow a reaction-limited process, while longer nanotubes ( 300 nm) adsorbed much more slowly, consistent with adsorption partially influenced by diffusion-limited processes. Finally, nanotubes were stably dispersed in river water and their adsorption onto a collagen layer was measured. The result was that smaller amounts of nanotubes were adsorbed onto the collagen layer compared to the adsorption under HEPES buffer; it is likely that passivation of the collagen layer by dissolved organic species could thus affect the end distribution of released nanotubes in a natural environment.
, Fagan, J.
, Huh, J.
, Migler, K.
, Karim, A.
and Raghavan, D.
lSPR Study of DNA Wrapped Single Wall Carbon Nanotube (ssDNA-SWCNT) Adsorption on a Model Biological (Collagen) Substrate, Soft Matter, [online], https://doi.org/10.1039/C0SM00368A
(Accessed December 11, 2023)