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Liquid Chromatography with Isotope-Dilution Mass Spectrometry for Determination of Water-Soluble Vitamins in Foods

Published

Author(s)

Melissa M. Phillips

Abstract

Vitamins are essential for improving and maintaining human health, and the main source of vitamins in the diet. Measurement of the quantities of water-soluble vitamins in common food materials is important to understand the impact of vitamin intake on human health, and also to provide necessary information for regulators to determine adequate intakes. Liquid chromatography (LC) and mass spectrometry (MS) based methods for water-soluble vitamin analysis are abundant in the literature, but most focus on only fortified foods or dietary supplements or allow determination of only a single vitamin. In this work, a method based on LC/MS and LC/MS/MS has been developed to allow simultaneous quantitation of eight water-soluble vitamins, including multiple forms of vitamins B3 and B6, in a variety of fortified and unfortified food-matrix Standard Reference Materials (SRMs). Thorough optimization of the vitamin extraction and confirmation using data from external laboratories ensured accuracy in the assigned values, and addition of stable isotope labeled internal standards for each of the vitamins allowed for increased precision.
Citation
Analytical Chemistry

Keywords

vitamins, nutrition, fortification, food, mass spectrometry, isotope dilution

Citation

Phillips, M. (2014), Liquid Chromatography with Isotope-Dilution Mass Spectrometry for Determination of Water-Soluble Vitamins in Foods, Analytical Chemistry, [online], https://doi.org/10.1007/s00216-014-8354-y (Accessed July 18, 2024)

Issues

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Created November 30, 2014, Updated November 10, 2018