Phillips, M.
, Bedner, M.
, Reitz, M.
, Burdette, C.
, Nelson, M.
, Yen, J.
, Sander, L.
and Rimmer, C.
(2016),
Liquid Chromatography with Absorbance Detection and with Isotope-Dilution Mass Spectrometry for Determination of Isoflavones in Soy Standard Reference Materials, Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-016-9997-7
(Accessed April 24, 2024)
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Liquid Chromatography with Absorbance Detection and with Isotope-Dilution Mass Spectrometry for Determination of Isoflavones in Soy Standard Reference Materials
Published
Author(s)
, , Manuela Reitz, , , , ,
Abstract
Two independent analytical approaches, based on liquid chromatography with absorbance detection and liquid chromatography with mass spectrometric detection, have been developed for determination of isoflavones in soy materials. These two methods yield comparable results for a variety of soy-based foods and dietary supplements. Four Standard Reference Materials (SRMs) have been produced by the National Institute of Standards and Technology to assist the food and dietary supplement community in method validation and have been assigned values for isoflavone content using both methods. These SRMs include SRM 3234 Soy Flour, SRM 3236 Soy Protein Isolate, SRM 3237 Soy Protein Concentrate, and SRM 3238 Soy-Containing Solid Oral Dosage Form. A fifth material, SRM 3235 Soy Milk, was evaluated using the methods and found to be inhomogeneous for isoflavones and unsuitable for value assignment.Citation
Analytical and Bioanalytical Chemistry
Pub Type
Journals
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Keywords
isoflavones, soy, reference material
Citation
Created November 10, 2016, Updated November 10, 2018