Liquid Chromatography with Absorbance Detection and with Isotope-Dilution Mass Spectrometry for Determination of Isoflavones in Soy Standard Reference Materials

Published

November 10, 2016

Author(s)

Melissa M. Phillips, Mary Bedner, Manuela Reitz, Carolyn Q. Burdette, Michael A. Nelson, James H. Yen, Lane C. Sander, Catherine A. Rimmer

Abstract

Two independent analytical approaches, based on liquid chromatography with absorbance detection and liquid chromatography with mass spectrometric detection, have been developed for determination of isoflavones in soy materials. These two methods yield comparable results for a variety of soy-based foods and dietary supplements. Four Standard Reference Materials (SRMs) have been produced by the National Institute of Standards and Technology to assist the food and dietary supplement community in method validation and have been assigned values for isoflavone content using both methods. These SRMs include SRM 3234 Soy Flour, SRM 3236 Soy Protein Isolate, SRM 3237 Soy Protein Concentrate, and SRM 3238 Soy-Containing Solid Oral Dosage Form. A fifth material, SRM 3235 Soy Milk, was evaluated using the methods and found to be inhomogeneous for isoflavones and unsuitable for value assignment.

Citation

Analytical and Bioanalytical Chemistry

Pub Type

Journals

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DOI Link

Keywords

isoflavones, soy, reference material

Citation

Phillips, M. , Bedner, M. , Reitz, M. , Burdette, C. , Nelson, M. , Yen, J. , Sander, L. and Rimmer, C. (2016), Liquid Chromatography with Absorbance Detection and with Isotope-Dilution Mass Spectrometry for Determination of Isoflavones in Soy Standard Reference Materials, Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-016-9997-7 (Accessed December 14, 2024)