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INTERLABORATORY TRIAL FOR MEASUREMENT OF VITAMIN D AND 25(OH)D IN FOODS AND A DIETARY SUPPLEMENT USING LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Published

Author(s)

Janet M. Roseland, Kristine Y. Patterson, Karen Andrews, Katherine M. Phillips, Melissa M. Phillips, Pamela R. Pehrsson, Guy L. Dufresne, Pavel A. Gusev, Sushma Savarala, Quynhanh V. Nguyen, Andrew J. Makowski, Guillaume P. Larouche, Stephen A. Wise, J. Harnly, Juhi R. Williams, Joseph M. Betz, Christine Taylor

Abstract

Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the U.S. and other countries participated, all using liquid chromatograph tandem-mass spectrometry but no common analytical protocol. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Other than one material which had vitamin D3 content close to quantitation limits, reproducibility results for the materials were acceptable. Thus it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.
Citation
Journal of Agricultural and Food Chemistry

Keywords

Vitamin D, 25-hydroxyvitamin D, 25(OH)D, reference material, analytical, food, dietary supplement, SRM 1577c Bovine Liver, SRM 1546a Meat Homogenate, SRM 1845a Whole Egg Powder
Created April 5, 2016, Updated November 10, 2018