NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.
Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.
An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B
Published
Author(s)
Atul Bhangale, Santanu S. Kundu, Wenchun Xie, William E. Wallace, Kathleen M. Flynn, Kathryn L. Beers, Richard Gross
Abstract
Epoxy functionalized supports are known to be suitable for industrial-scale immobilization of enzymes for biocatalysis. However, chemistry across these epoxy groups is known to affect the catalytic performance of the enzymes.In order to achieve a balance between immobilized enzyme activity and minimize enzyme leaching, immobilization supports with different physico-chemical properties were systematically studied. In the present study, we report immobilization of CAL B onto epoxy functionalized supports. CAL B was immobilized covalently on epoxy functionalized supports with different physico- chemical properties. Amongst them, Amberzyme and silanized silica support were selected as potential candidates and were studied in detail. In parallel, a microreactor based approach was developed in order to more systematically quantify the behavior of solid-supported catalysts in a continuous flow environment. Results indicates that covalently immobilized CAL B on silanized silica support showed better performance than that observed for methacrylate support, but lower biocatalytic performance as compared to physically immobilized CAL B on PMMA support.
Proceedings Title
American Chemical Society Spring 2010 National Meeting
Bhangale, A.
, Kundu, S.
, Xie, W.
, Wallace, W.
, Flynn, K.
, Beers, K.
and Gross, R.
(2010),
IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B, American Chemical Society Spring 2010 National Meeting , San Francisco, CA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=904144
(Accessed October 11, 2025)