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IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B

Published

Author(s)

Atul Bhangale, Santanu S. Kundu, Wenchun Xie, William E. Wallace, Kathleen M. Flynn, Kathryn L. Beers, Richard Gross

Abstract

Epoxy functionalized supports are known to be suitable for industrial-scale immobilization of enzymes for biocatalysis. However, chemistry across these epoxy groups is known to affect the catalytic performance of the enzymes.In order to achieve a balance between immobilized enzyme activity and minimize enzyme leaching, immobilization supports with different physico-chemical properties were systematically studied. In the present study, we report immobilization of CAL B onto epoxy functionalized supports. CAL B was immobilized covalently on epoxy functionalized supports with different physico- chemical properties. Amongst them, Amberzyme and silanized silica support were selected as potential candidates and were studied in detail. In parallel, a microreactor based approach was developed in order to more systematically quantify the behavior of solid-supported catalysts in a continuous flow environment. Results indicates that covalently immobilized CAL B on silanized silica support showed better performance than that observed for methacrylate support, but lower biocatalytic performance as compared to physically immobilized CAL B on PMMA support.
Proceedings Title
American Chemical Society Spring 2010 National Meeting
Volume
51
Issue
1
Conference Dates
March 21-25, 2010
Conference Location
San Francisco, CA

Keywords

Microfluidics, CAL B, immobilization

Citation

Bhangale, A. , Kundu, S. , Xie, W. , Wallace, W. , Flynn, K. , Beers, K. and Gross, R. (2010), IMPACT OF IMMOBILIZATION SUPPORTS FOR POLYESTERS SYNTHESIS ACTIVITY OF CANDIDA ANTARTICA LIPASE B, American Chemical Society Spring 2010 National Meeting , San Francisco, CA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=904144 (Accessed April 21, 2024)
Created January 1, 2010, Updated February 19, 2017