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Hydrodynamic chromatography



Andre M. Striegel, Amandaa Brewer


Hydrodynamic chromatography (HDC) has experienced a resurgence in recent years for particle and polymer characterization due, principally, to its coupling to a multiplicity of physical detection methods. Coupled to light scattering (both multi-angle static and quasi-elastic), viscometric, and refractometric detectors, HDC has the ability to determine the molar mass, size, shape, and structure of colloidal analytes continuously and as a function of one another, all in a single analysis and exposing the analytes to less shear forces (hence, less potential for flow-induced degradation) than in e.g., size-exclusion chromatography. Here, the fundamental chromatographic underpinnings of the technique are reviewed in terms of retention, band broadening, and resolution, and the power of multi-detector HDC is showcased using examples from the recent literature.
Annual Review of Analytical Chemistry


Hydrodynamic chromatography, multiple detection, particle sizing, polymer characterization, chromatography fundamentals, packed column HDC, microcapillary HDC


Striegel, A. and Brewer, A. (2012), Hydrodynamic chromatography, Annual Review of Analytical Chemistry (Accessed April 21, 2024)
Created June 21, 2012, Updated February 19, 2017