We present the development and evaluation of an engineered hybrid cell adhesive surface for instant dielectrophoretic trapping and patterning of cells in a microfluidic device. This work demonstrates that a hybrid cell adhesive material (hCAM) prepared from fibronectin and a polyallylamine hydrochloride (PAH) layer on top of polyelectrolyte multilayers (PEMs) can successfully allow pluripotent P19 cells to be cultured and induced to differentiate into neuron-like cells within the controlled environment of a microfluidic device. P19 cells were cultured for two days prior to the induction process that takes four days. After the induction step differentiated P19 cells were observed on the 8th day after the DEP manipulation was carried out. Staining for neurofilaments, a neural differentiation marker, showed neuronal processes extending from the cell bodies of cells patterned on the DEP electrodes. This system provides a microfluidics tool for the instantaneous trapping of cells for rapid return to cell growth conditions and assessment of long-term cellular processes such as cell proliferation, induction and differentiation.
Pub Type: Journals
dielectrophoresis, cell differentiation, cell adhesive material, long-term cell studies, fibronectin, polyelectrolytes