Homogenization of Large Batch Frozen Materials Using the Palla-VM-KT Vibrating Mill

Published

May 21, 2024

Author(s)

Debra Ellisor, Amanda Moors, Jennifer Hoguet, Jennifer Ness, Amanda Capuano, Olivia Franco

Abstract

The Biospecimen Science Group at the National Institute of Standards and Technology (NIST) Biorepository has been responsible for the curation, maintenance, and preparation of biological specimens at cryogenic temperatures since 2002. The team's expertise in cryohomogenization was leveraged to lead NIST's efforts in generating large batches of homogenous, fresh-frozen materials using a Palla VM-KT Vibrating mill. Most of these materials are part of the NIST SRM (Standard Reference Material) program, aiding collaborators and stakeholders in verifying the accuracy of measurements, calibrating instruments, and/or supporting the development of new measurement methods. Collaborating institutions can also request cryohomogenization of matrices for their own specific use. This report outlines important considerations and required preparations prior to cryomilling and describes the procedure for operating the cryomill at the NIST Biorepository.

Citation

Special Publication (NIST SP) - 1196r1

Report Number

1196r1

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.1196r1

Local Download

Keywords

biorepository, cryohomogenization, reference material.

Citation

Ellisor, D. , Moors, A. , Hoguet, J. , Ness, J. , Capuano, A. and Franco, O. (2024), Homogenization of Large Batch Frozen Materials Using the Palla-VM-KT Vibrating Mill, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1196r1, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=957773 (Accessed June 15, 2024)