High-Throughput Genotyping of Short Tandem Repeat DNA Markers With Time-of-Flight Mass Spectrometry
John M. Butler
Time-of-flight mass spectrometry (TOF-MS) combined with matrix-assisted laser desorption/ionization (MALDI) can be used to rapidly genotype short tandem repeat (STR) DNA markers. This article reviews various applications and conventional approaches for analysis of STRs and contrasts electrophoretic DNA separation methods with mass spectrometric ones. There is a focus on the advantages and challenges of using MALDI-TOF-MS for reliable genotyping of STRs. Different approaches for reducing DNA size and sample salt content prior to mass spectrometric analysis are discussed as are issues surrounding high volume sample processing such as factors impacting the speed of rapid MS data collection.
Encyclopedia of Mass Spectrometry
high-throughput genotyping, large DNA molecules, MALDITOF-MS, PCR product analysis, sample salt reduction, short tandem repeats
High-Throughput Genotyping of Short Tandem Repeat DNA Markers With Time-of-Flight Mass Spectrometry, Encyclopedia of Mass Spectrometry
(Accessed November 29, 2023)