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High-Throughput Genotyping of Short Tandem Repeat DNA Markers With Time-of-Flight Mass Spectrometry

Published

Author(s)

John M. Butler

Abstract

Time-of-flight mass spectrometry (TOF-MS) combined with matrix-assisted laser desorption/ionization (MALDI) can be used to rapidly genotype short tandem repeat (STR) DNA markers. This article reviews various applications and conventional approaches for analysis of STRs and contrasts electrophoretic DNA separation methods with mass spectrometric ones. There is a focus on the advantages and challenges of using MALDI-TOF-MS for reliable genotyping of STRs. Different approaches for reducing DNA size and sample salt content prior to mass spectrometric analysis are discussed as are issues surrounding high volume sample processing such as factors impacting the speed of rapid MS data collection.
Citation
Encyclopedia of Mass Spectrometry

Keywords

high-throughput genotyping, large DNA molecules, MALDITOF-MS, PCR product analysis, sample salt reduction, short tandem repeats

Citation

Butler, J. (2008), High-Throughput Genotyping of Short Tandem Repeat DNA Markers With Time-of-Flight Mass Spectrometry, Encyclopedia of Mass Spectrometry (Accessed May 17, 2024)

Issues

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Created October 16, 2008