High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

Published: July 20, 2014

Author(s)

Charles H. Camp, Young J. Lee, John M. Heddleston, Christopher M. Hartshorn, Angela R. Hight Walker, Jeremy N. Rich, Justin D. Lathia, Marcus T. Cicerone

Abstract

We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3000 cm−1 and generates an especially strong response in the typically weak Raman “fingerprint” region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of < 13 cm−1. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter.
Citation: Nature Photonics
Volume: 8
Pub Type: Journals

Keywords

broadband coherent anti-Stokes Raman scattering, chemical imaging, coherent anti-Stokes Raman scattering, hyperspectral imaging, label-free
Created July 20, 2014, Updated February 19, 2017