High Pressure Cell for Bio-SANS Studies under Sub-Zero Temperatures or Heat Denaturing Conditions

Published

June 28, 2018

Author(s)

Susana Cristina Marujo Teixeira, Juscelino Leao, Cedric Victor Lucien Gagnon, M. A. McHugh

Abstract

The second biennial International Society for Sample Environment (ISSE) school included training on high pressure (HP) cells for Soft Matter studies. Neutron research centers are responding to a growing need for HP experimental data, relevant to broad range of applications in bioscience, biotechnology and others. The need to disseminate existing designs and operating protocols, to support concerted efforts towards the advancement of sample environment instrumentation across research centers, was highlighted throughout discussions during the ISSE school. The liquid insertion pressure system for SANS (LIPSS), currently used at the NIST Center for Neutron Research for solutions of biological macromolecules, is described here. Emphasis is placed on the context of subzero temperature studies and the design challenges.

Citation

Journal of Neutron Research

Volume

20

Issue

1

Pub Type

Journals

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Keywords

High pressure, subzero temperature, Small-Angle Neutron Scattering, Cold Denaturation, Proteins

Citation

, S. , Leao, J. , Lucien, C. and , M. (2018), High Pressure Cell for Bio-SANS Studies under Sub-Zero Temperatures or Heat Denaturing Conditions, Journal of Neutron Research, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=925308 (Accessed October 10, 2025)