A cell co-culture was generated on opposite sides of a permeable polyester membrane (PET) membrane by combining dielectrophoretic (DEP) and electrostatic forces to hold cells against gravity in a multilayer microfluidic device (Figure 1). HepG2 cells were trapped underneath the PET membrane (bottom channel) when DEP attractive forces were applied. Cells immediately adhered on the PET membrane by the electrostatic forces from a hybrid cell adhesive material (hCAM) deposited on the surface of the electrodes. NIH-3T3 cells adhered on the top channel via the hCAM deposited on that side of the membrane. Cells on both sides of the membrane remained attached and viable at least 24 h after the co-culture was generated.
Proceedings Title: Proceedings of Micro Total Analysis Systems 2012
Conference Dates: October 28-November 1, 2012
Conference Location: Okinawa, -1
Conference Title: The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pub Type: Conferences
Dielectrophoresis, cell co-cultures, polyester (PET) membrane, fibronectin, polyelectrolyte multilayers