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A Generalized Strategy for Immobilizing Uniformly Oriented Membrane Proteins at Solid Interfaces

Published

Author(s)

Amit NMN Vaish, Vitalii I. Silin, Marlon L. Walker, Kristen L. Steffens, Susan Krueger, Alexei A. Yeliseev, Klaus Gawrisch, David J. Vanderah

Abstract

We present here a generalized strategy for immobilizing uniformly oriented membrane proteins onto a functionalized surface. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.
Citation
Chemical Communications
Volume
49

Keywords

SAM, GPCR, SPR, Neutron Scattering, membrane protein, XPS

Citation

, A. , Silin, V. , Walker, M. , , K. , Krueger, S. , , A. , Gawrisch, K. and Ross, D. (2013), A Generalized Strategy for Immobilizing Uniformly Oriented Membrane Proteins at Solid Interfaces, Chemical Communications, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=913072 (Accessed June 13, 2024)

Issues

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Created February 13, 2013, Updated February 19, 2017