Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Further Studies with Isolated Absolute Infrared Spectra of Bacteriorhodopsin Photocycle Intermediates: Conformational Changes and Possible Role of a New Proton-Binding Center

Published

Author(s)

Richard W. Hendler, Curtis W. Meuse, Paul Smith, John W. Kakareka

Abstract

We recently published procedures describing the isolation of absolute infrared spectra for the intermediates of the bacteriorhodopsin (BR) photocycle and from these, obtaining transitional difference spectra between consecutive intermediates. In that work, we concentrated mainly on proton-binding centers and the route of proton transport across the membrane. In the current study, we used isolated spectra for the amide I, amide II, and amide III envelopes to obtain quantitative information on the extent of conformational change accompanying each transition in the photocycle. Our main finding was that most of the conformational changes occur in the conversion of the MF intermediate to N. In our earlier publication, a new proton acceptor, absorbing at 1650 cm1 was identified, which appeared to accept a proton from Asp96COOH during the transformation of BR to L. Below, we present evidence that supports this interpretation and propose a possible role for this new component.
Citation
Applied Spectroscopy
Volume
67
Issue
1

Keywords

Conformational changes, FT-IR kinetics, Amides I, II, and III.

Citation

Hendler, R. , Meuse, C. , Smith, P. and Kakareka, J. (2013), Further Studies with Isolated Absolute Infrared Spectra of Bacteriorhodopsin Photocycle Intermediates: Conformational Changes and Possible Role of a New Proton-Binding Center, Applied Spectroscopy, [online], https://doi.org/10.1366/12-06662 (Accessed December 3, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created January 1, 2013, Updated November 10, 2018