Fluorescence in Situ Hybridization (FISH) Methods for Medical Genetics; Proposed Guideline

Published

May 1, 2002

Author(s)

Peter E. Barker, R K. Enns, G Dewalk, D J. Rasmussen, E Shulse, M S. Watson, P R. Wyatt

Abstract

FISH technology can be used to detect microdeletions that are not visible by standard cytogenetic banding patterns. FISH technology allows for the rapid determination of whether specific genes, loci, or regions are present or if deletions or amplifications have occurred. The size of these regions detected by FISH are usually much larger than detectable by PCR or Southern blot, yet much smaller then visualized microscopically by standard cytogenetics. This uniquie ability of FISH technology provides strong justification for its diagnostic use in cancer, prenatal, postnatal, and other genetic diseases. Since FISH is rapidly becoming a routine method used in medical genetics laboratories, a NCCLS guideline will help ensure its appropriate and reliable use in these laboratories.

Citation

NCCLS Guidelines

Volume

21

Issue

No. 16

Pub Type

Journals

Keywords

FISH

Citation

Barker, P. , Enns, R. , Dewalk, G. , Rasmussen, D. , Shulse, E. , Watson, M. and Wyatt, P. (2002), Fluorescence in Situ Hybridization (FISH) Methods for Medical Genetics; Proposed Guideline, NCCLS Guidelines (Accessed October 21, 2025)