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Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating

Published

Author(s)

Pierre-Alain Auroux, Darwin R. Reyes-Hernandez, Jayna J. Shah, Michael Gaitan

Abstract

We report 100% yield and 40% reaction-time reduction for on-chip DNA amplification using microwave dielectric heating. A passivation protocol has also been developed to suppress the inhibition of PCR in PMMA chips used with a temperature sensor. We finally compare the reaction time of the on-chip microwave PCR amplification and the one of a conventional thermocycler.
Proceedings Title
Miniaturized Systems for Chemistry and Life Sciences
Conference Dates
October 7-11, 2007
Conference Location
Paris, FR
Conference Title
Micro Total Analytical Systems (uTAS) Conference

Keywords

DNA, microwave dielectric heating, PCR, thermocycling

Citation

Auroux, P. , Reyes-Hernandez, D. , Shah, J. and Gaitan, M. (2007), Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating, Miniaturized Systems for Chemistry and Life Sciences, Paris, FR (Accessed April 16, 2024)
Created October 15, 2007, Updated January 27, 2020