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PUBLICATIONS

Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating

Published

Author(s)

Pierre-Alain Auroux, Darwin R. Reyes-Hernandez, Jayna J. Shah, Michael Gaitan

Abstract

We report 100% yield and 40% reaction-time reduction for on-chip DNA amplification using microwave dielectric heating. A passivation protocol has also been developed to suppress the inhibition of PCR in PMMA chips used with a temperature sensor. We finally compare the reaction time of the on-chip microwave PCR amplification and the one of a conventional thermocycler.
Proceedings Title
Miniaturized Systems for Chemistry and Life Sciences
Conference Dates
October 7-11, 2007
Conference Location
Paris, FR
Conference Title
Micro Total Analytical Systems (uTAS) Conference
Pub Type
Conferences

Keywords

DNA, microwave dielectric heating, PCR, thermocycling

Citation

Auroux, P. , Reyes-Hernandez, D. , Shah, J. and Gaitan, M. (2007), Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating, Miniaturized Systems for Chemistry and Life Sciences, Paris, FR (Accessed October 12, 2025)

Issues

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Created October 15, 2007, Updated January 27, 2020
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