Auroux, P.
, Reyes-Hernandez, D.
, Shah, J.
and Gaitan, M.
(2007),
Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating, Miniaturized Systems for Chemistry and Life Sciences, Paris, FR
(Accessed September 19, 2024)
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Fast and High Efficient Microfluidic PCR By Microwave Dielectric Heating
Published
Author(s)
, , ,
Abstract
We report 100% yield and 40% reaction-time reduction for on-chip DNA amplification using microwave dielectric heating. A passivation protocol has also been developed to suppress the inhibition of PCR in PMMA chips used with a temperature sensor. We finally compare the reaction time of the on-chip microwave PCR amplification and the one of a conventional thermocycler.Proceedings Title
Miniaturized Systems for Chemistry and Life Sciences
Conference Dates
October 7-11, 2007
Conference Location
Paris, FR
Conference Title
Micro Total Analytical Systems (uTAS) Conference
Pub Type
Conferences
Keywords
DNA, microwave dielectric heating, PCR, thermocycling
Citation
Issues
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Created October 15, 2007, Updated January 27, 2020