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Evolution and Impact of Standard Reference Materials (SRMs) for the Determining Vitamin D Metabolites



Stephen Wise, Adam Kuszak, Johanna Camara


The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health, Office of Dietary Supplements (NIH ODS), introduced the first Standard Reference Material® (SRM) for determining vitamin D metabolites in 2009 motivated by significant concerns about the comparability and accuracy of different assays to assess vitamin D status. After 14 years, a suite of five serum matrix SRMs and three calibration solution SRMs are available. Values were also assigned for vitamin D metabolites in five additional SRMs intended primarily to support measurements of other clinical diagnostic markers. Both the SRMs and the certification approach have evolved from significant exogenous serum content to primarily endogenous content and from value assignment by combining the results of multiple analytical methods to the use of measurements exclusively from reference measurement procedures (RMPs). The impact of the availability of these SRMs can be assessed by both the distribution information (sales) and by reports in the scientific literature describing their use for method validation, quality control, and research. In this review, we describe the development of these SRMs, the evolution in design and value assignment, the expansion of information reported, and SRM use in validating analytical methods and providing quality assurance within the vitamin D measurement community.
Analytical and Bioanalytical Chemistry


25-hydroxyvitamin D2, 25-hydroxyvitamin D3, total 25-hydroxyvitamin D, 3-epi-25-Hydroxyvitamin D3, Vitamin D binding protein (VDBP), Reference measurement procedure (RMP)


Wise, S. , Kuszak, A. and Camara, J. (2024), Evolution and Impact of Standard Reference Materials (SRMs) for the Determining Vitamin D Metabolites, Analytical and Bioanalytical Chemistry, [online],, (Accessed March 1, 2024)
Created January 18, 2024