Evaluation of Method-Specific Extraction Variability for the Measurement of Fatty Acids in a Candidate Infant/Adult Nutritional Formula Reference Material
Benjamin J. Place
To address community needs, the National Institute of Standards and Technology (NIST) has developed a candidate Standard Reference Material (SRM) for infant/adult nutritional formula based on milk and whey protein concentrates with isolated soy protein: SRM 1869 Infant/Adult Nutritional Formula. One major component of the candidate SRM is the fatty acid content. In this study, multiple extraction techniques were evaluated to quantify the fatty acids in the new material. Extraction methods that were based on lipid extraction followed by transesterification resulted in lower mass fraction values for all fatty acids than the values measured by methods utilizing in situ transesterification followed by fatty acid methyl ester extraction (ISTE). A final method, based on the developed ISTE protocols, was used to determine the fatty acid content of the new infant/adult nutritional formula reference material.
Evaluation of Method-Specific Extraction Variability for the Measurement of Fatty Acids in a Candidate Infant/Adult Nutritional Formula Reference Material, Journal of Aoac International, [online], https://doi.org/10.5740/jaoacint.16-0356
(Accessed May 28, 2023)