Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips

Published

Author(s)

Darwin R. Reyes-Hernandez

Abstract

An approach that combines not only the cell manipulation capabilities of dielectrophoresis (DEP), but also the conditions to promote appropriate cell function during long-term experiments is presented. The integration of a hybrid cell adhesive material (hCAM) with the trapping forces of positive DEP have demonstrated that after DEP trapping and anchorage on the hCAM cells can be differentiated into neuron-like cells after more than a week in the microfluidic system. This approach has also shown promise in the development of a multilayer microfluidic device, where cells can be trapped on opposite sides of a permeable polyester (PET) membrane that separates two channels running perpendicular to each other. Within these devices cells can be exposed to the specific microenvironments required for appropriate cell co-culture conditions. This system can be used for studies such as cell-cell communication, cell migration and many others.
Proceedings Title
PRIME, Pacific RIM Meeting on Electrochemical and Solid-State Science
Conference Dates
October 7-12, 2012
Conference Location
Honolulu, HI
Conference Title
Electrochemical Society 222nd Meeting

Citation

Reyes-Hernandez, D. (2012), Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips, PRIME, Pacific RIM Meeting on Electrochemical and Solid-State Science, Honolulu, HI (Accessed May 25, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created October 7, 2012, Updated February 19, 2017