Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips
Darwin R. Reyes-Hernandez
An approach that combines not only the cell manipulation capabilities of dielectrophoresis (DEP), but also the conditions to promote appropriate cell function during long-term experiments is presented. The integration of a hybrid cell adhesive material (hCAM) with the trapping forces of positive DEP have demonstrated that after DEP trapping and anchorage on the hCAM cells can be differentiated into neuron-like cells after more than a week in the microfluidic system. This approach has also shown promise in the development of a multilayer microfluidic device, where cells can be trapped on opposite sides of a permeable polyester (PET) membrane that separates two channels running perpendicular to each other. Within these devices cells can be exposed to the specific microenvironments required for appropriate cell co-culture conditions. This system can be used for studies such as cell-cell communication, cell migration and many others.
PRIME, Pacific RIM Meeting on Electrochemical and Solid-State Science
Enabling Long-Term Dielectrophoretic Actuation for Cell Manipulation and Analysis in Microfluidic Biochips, PRIME, Pacific RIM Meeting on Electrochemical and Solid-State Science, Honolulu, HI
(Accessed December 4, 2023)