Elevated urinary levels of 8-hydroxy-2-deoxyguanosine, (5R)- and (5S)-8,5-cyclo-2- deoxyadenosines, and 8-iso-prostaglandin F2¿ as potential biomarkers of oxidative stress in patients with prediabetes
M Miral Dizdar, Melis Kant, Merve Akis, Mehmet Calan, Tugba Arkan, Firat Bayraktar, Huray Islekel
Prediabetes is the preclinical stage of type 2 diabetes mellitus with intermediate state of hyperglycemia. Hyperglycemia results in a state of oxidative stress and that reactive oxygen species contribute to the production of insulin resistance, β-cell dysfunction and long- term complications of diabetes. Novel approaches are required for prevention and treatment diabetes. New biomarkers that can be used in risk stratification and therapy control as supplementary to current parameters are needed. These biomarkers may facilitate a more individualized and sufficient treatment of diabetes. Therefore, the aim of this study was to investigate the levels of oxidatively induced DNA damage, 8-hydroxy-2'-deoxyguanosine (8-OH- dG), (5'R)- and (5'S)-8,5'-cyclo-2'-deoxyadenosines (R-cdA and S-cdA), and the lipid peroxidation product 8-iso-prostaglandin F2α (8-iso-PGF2α) as reliable oxidative stress markers in patients with prediabetes, type 2 diabetes mellitus (T2DM) in comparison with healthy volunteers. Urine samples were collected from prediabetes (n = 47) and T2DM (n = 43) patients, and healthy volunteers (n = 37). Absolute quantification of 8-OH-dG, R-cdA, S-cdA and 8-iso-PGF2α was achieved by liquid chromatography-isotope dilution tandem mass spectrometry (LC-MS/MS). The levels of 8-OH-dG, S-cdA and 8-iso-PGF2α were significantly greater in prediabetes patients than those in healthy volunteers (p
, Kant, M.
, Akis, M.
, Calan, M.
, Arkan, T.
, Bayraktar, F.
and Islekel, H.
Elevated urinary levels of 8-hydroxy-2’-deoxyguanosine, (5’R)- and (5’S)-8,5’-cyclo-2’- deoxyadenosines, and 8-iso-prostaglandin F2¿ as potential biomarkers of oxidative stress in patients with prediabetes, Dna Repair, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=921067
(Accessed October 1, 2023)