Efficient Synthesis and HPLC-Based Characterization for Developing Vanadium-48-Labeled Vanadyl Acetylacetonate as a Novel Cancer Radiotracer for PET Imaging

Published

February 9, 2024

Author(s)

Brittany Broder, Mohammed Bhuiyan, Richard Freifelder, David Rotsch, Satish Chitneni, Marvin William Makinen, Chin-Tu Chen

Abstract

Bis(acetylacetonato)oxidovanadium(IV) [(VO(acac)2], generally known as vanadyl acetylacetonate, has been shown to be preferentially sequestered in malignant tissue. Vanadium-48 (48V) generated with a compact medical cyclotron has been used to label VO(acac)2 as a potential radiotracer in positron emission tomography (PET) imaging for the detection of cancer, but requires lengthy synthesis. Current literature protocols for the characterization of VO(acac)2 require macroscale quantities of reactants and solvents to identify products by color and to enable crystallization that are not readily adaptable to the needs of radiotracer synthesis. We present an improved method to produce vanadium-48-labeled VO(acac)2, [48V]VO(acac)2, and characterize it using high-performance liquid chromatography (HPLC) with radiation detection in combination with UV detection. The approach is suitable for radiotracer-level quantities of material. These methods are readily applicable for production of [48V]VO(acac)2. Preliminary results of preclinical, small-animal PET studies are presented.

Citation

MDPI Molecules

Volume

29

Issue

4

Pub Type

Journals

Download Paper

https://doi.org/10.3390/molecules29040799

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Keywords

vanadium, vanadium-48, vanadyl acetylacetonate, high performance liquid chromatography (HPLC), positron emission tomography (PET), PET imaging, radioactivity

Citation

Broder, B. , Bhuiyan, M. , Freifelder, R. , Rotsch, D. , Chitneni, S. , Makinen, M. and Chen, C. (2024), Efficient Synthesis and HPLC-Based Characterization for Developing Vanadium-48-Labeled Vanadyl Acetylacetonate as a Novel Cancer Radiotracer for PET Imaging, MDPI Molecules, [online], https://doi.org/10.3390/molecules29040799, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=957089 (Accessed April 27, 2024)